Super-Resolution Fluorescence Microscopy by Single-Molecule Switching
Super-Resolution Fluorescence Microscopy by Single-Molecule Switching Biological research has been greatly impacted by the invention of fluorescent microscopy (Thorley, Pike and Rappoport, 2014). Although the impact on describing biological processes is revolutionary, diffraction of light is a limiting factor that blurs objects smaller than 250 nm in the x and y direction, and 500 nm in the z direction. Cellular structures, including ribosomes and action […]