Lab: The Bacteria Around You James Brunet Ms Owen October 14th, 2012 Part 1 Purpose To culture and observe the various types of bacteria found around Canterbury High School. Materials and Methods Refer to pages 422-425 of Biology 11 McGraw-Hill Ryerson and the handout “Gram Staining Procedure”. Observations Table 1: Locations of Bacteria Samples Quadrant| Location of Sample Obtained| 1| Floor| 2| Water fountain head| […]

Different types of bacteria in various forms are found all around us, and it is a microbiologist’s job to be able to identify these bacteria. Using various staining techniques and physiological tests, an isolated bacterium can be identified. In this experiment, a single bacterial colony was isolated form Mycorrhizal spores, and further tests done on that colony. Sub culturing was done after each week to […]

Infection are spread through different means : Stool Contact with blood Sneezing and coughing Airborne disease Contaminated objects Direct physical contact The methods to reduce the spread of infection Make sure that everyone clean their hand before and after having the meal, visiting the toilet and changing nappies with soap and water Make sure that all the toys and quipment used for child are safe […]

Abstract Some bacteria are able to go through transformation making new combinations of genes. Transformation is a way of gene variability in bacteria. This experiment is based on the transformation mechanism of bacteria and gene regulation. The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLO™ plasmid. After the insertion of the […]

Abstract Four pure, unidentified cultures of (gram positive cocci) bacteria, labelled A-D were cultured on various agar media. Also an API test was simulated to identify another unidentified bacterium. Identification of bacteria is important when choosing an effective treatment for a microbial-causing illness. This experiment focused on the cultural and biochemical characteristics of bacteria in aid of identification. Under aseptic conditions, each of the four […]

An attack of bacterial meningitis can be devastating because most of its victims are usually children or infants. The symptoms of the disease can also be misread leading to improper or inadequate treatment with fatal consequences. As risks of misdiagnosis are many, it is recommended that the services of a physician be sought as quickly as the first symptoms are noticed. Meningitis is the disease […]

Several Escherichia coli (ATCC 11229) bacterial cultures were established using serial dilutions and simple agar plate pouring. The growth of the bacterial cultures was evaluated using spectrophotometric and colony counting methods. Data collected from a two-hour monitoring using 30-minute interval sampling of bacterial suspensions were plotted against the 30-minute interval collection times on a normal and semilographic coordinates. The resulting growth curves showed that the […]

Students were assigned unknown organisms and the objective of the lab tests was to identify of the unknown organisms. A microorganism can be identified as an organism that can be through the aid of the microscope. In a typical condition, microorganisms are said to contain cells that are in singular mode. A perfect example of such microorganisms is such as bacteria, fungi, some types of […]

Genetic transformation is a process that primarily is inserting new DNA into an organism to change that organism’s trait. This process has many useful benefits when used correctly in different organisms. In this lab, bacteria was transformed by inserting DNA for Green Fluorescent Proteins. The DNA for these proteins were taken from bioluminescent jellyfish Aequorea victoria. One of the main lessons of the lab is […]

INTRODUCTION The environments of Earth include conditions in which physical and chemical extremes make it very difficult for organisms to survive. Conditions that can destroy living cells and biomolecules include high and low temperatures; low amounts of oxygen and water; and high levels of salinity, acidity, alkalinity, and radiation. Examples of extreme environments on Earth are hot geysers and oceanic thermal vents, Antarctic sea ice, […]