The last reason Rosalie Is Ideal for research Is the fly size and manipulability, since we can easily control them combined with the previous reasons makes them ideal for research. In our experimental research we attempted to observe genetic changes in three generations of flies. We were given the second generation to observe the traits and record. Our hypothesis is if the drosophila are allowed to interbreed, then genetic variation will occur. Materials 1. Culture vial of wild-type Drosophila 2. Culture vial A or B or C 3. Spoilsport Alcohol 10%, mall 4. Camel’s hair brush 5.
Thermo-anesthetizes 6. Petri dish Drosophila vials ; labels with medium 7. 8. Fly morgue 9. Forceps Procedures Part A 1 . Thermally Immobilizers a vial of wild-type Drosophila. Your Instructor will demonstrate the proper Manipulation technique. 2. Observe the flies’ traits, particularly body features that distinguish males and females, eye color, and wing size and shape. Record your observations in Table 1 in the Analysis section. If, at any time during your observations, the flies begin to become active, re-immobilizers them according to your instructor’s directions. Part B 1.
Obtain a vial off prepared Drosophila cross. 2. Record the letter written on your vial In Table 2 In the Analysis sections to help you keep track of which cross you have received. TLS will lad In determining expected results, as well as allow your instructor to identify any problems you may be having and to help correct them. 3. Immobilizers the parental generation of your cross and observe the flies under a stereoscope’s. If, at any time during your observations, the flies begin to become active, re-immobilizers them according to your instructor’s directions. 4. Separate the males from the females.
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